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基于线粒体结构和功能的外源草酸缓解采后芒果冷害机制

批准号31701665 学科分类果品贮藏与保鲜 ( C200602 )
项目负责人李佩艳 负责人职称讲师 依托单位河南科技大学
资助金额25.00
万元
项目类别青年科学基金项目 研究期限2018 年 01 月 01 日 至
2020 年 12 月 31 日
中文主题词冷害;胁迫;草酸;线粒体;芒果
英文主题词Chilling injury;Stress;Oxalic acid;Mitochondria;Mango

摘要

中文摘要 冷敏型果实低温贮藏极易发生冷害,导致其品质劣变并缩短贮藏期。目前,草酸在果实贮藏保鲜中的作用已引起极大关注,草酸不仅能有效延缓采后果实成熟,而且能缓解低温胁迫下采后果实冷害的发生,但草酸缓解采后果实冷害的机制仍不清楚。前期研究发现草酸缓解采后芒果果实冷害与其维持较高的能量水平有关。线粒体是能量产生的重要场所,逆境胁迫下果实线粒体结构和功能与采后果实的冷害密切相关。为进一步从线粒体结构和功能角度阐明草酸缓解采后果实冷害机制,本研究以冷敏型果实芒果为试验材料,通过研究草酸处理对低温胁迫下采后芒果果实线粒体结构、线粒体蛋白质、线粒体脂肪酸、线粒体电子传递链的影响,进一步从线粒体结构和功能角度揭示草酸缓解采后芒果果实冷害机制,本研究可为控制采后芒果果实冷害、延长果实贮藏期提供理论一定的理论依据和技术支持。因此,本研究具有重要的理论意义和应用价值。
英文摘要 Chilling sensitive fruits are susceptible to chilling injury during storage at low temperature, which result in extremely decrease in storage period and fruit quality. Oxalic acid could not only effectively delay the ripening process in postharvest fruits, but also could alleviate chilling injury in chilling-storage fruits, which has attracted great attention in the studies of fruit storage and keeping fresh. However, the mechanism of oxalic acid alleviating chilling injury in postharvest fruits is still not clear. Preliminary study found that chilling injury in fruits was associated with keeping higher energy status. Mitochondria are an important place generating cellular energy, and the structure and function of mitochondria in fruits was involved in chilling injury under adversity stress. In order to further study the mechanism of oxalic acid alleviating chilling injury in postharvest fruits, mango as experimental material, the effect of oxalic acid.on the stucture, prtotein, fatty acid and mitochondrial electron transport chain were study in this research, which was conductive to illuminating the mechanism of oxalic acid allevating chilling injury of postharvest mango fruits from the perspective of mitochondria strucure and function. These study could provide fundamental basis for controlling chilling injury in postharvest mango fruit and prolonging fresh-storage period. Therefore, this research has both important theoretical and applied values.
结题摘要

成果

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